Pollen World
Pollen Biology Lab, Czech Academy of Sciences

Laboratory of Pollen Biology ASCR





Publications - 21st century





2017


Fíla J, Záveská Drábková L, Gibalová A, Honys D (2017) When Simple Meets Complex: Pollen and the -Omics. In: Gerhard Obermeyer, José Feijó (eds.) Pollen Tip Growth, From Biophysical Aspects to Systems Biology. Springer-Verlag Berlin, Heidelberg, ISBN 978-3-319-56644-3, DOI 10.1007/978-3-319-56645-0_10. PDF

Fíla J, Honys D (2017) Phosphoprotein Enrichment from Tobacco Mature Pollen Crude Protein Extract. Methods Mol Biol 1669: 265-274. doi: 10.1007/978-1-4939-7286-9_21. PDF

Gibalová A, Steinbachová L, Hafidh S, Bláhová V, Gadiou Z, Michailidis C, Műller K, Pleskot R, Dupláková N, Honys D (2016). Characterization of pollen-expressed bZIP protein interactions and the role of ATbZIP18 in the male gametophyte. Plant Reproduction. Online first. DOI 10.1007/s00497-016-0295-5. PDF

Honys D, Honysová B (2017) Vývoj pylu: od mikrosporocytu ke zralému pylovému zrnu.
Botanika 5: 4-6. Fulltext




2016


Hafidh D, Potěšil D, Fíla J, Čapková V, Zdráhal Z, Honys D (2016) Quantitative Proteomics of the Tobacco Pollen tube-Secretome Identifies Novel Pollen Tube Guidance Proteins Important for Fertilization.
Genome Biology 17: 81. doi:10.1186/s13059-016-0928-x. PDF

Fíla J, Radau S, Matros A, Hartmann A, Scholz U, Feciková J, Mock H-P, Čapková V, Peiman Zahedi R, Honys D (2016) Phosphoproteomics profiling of tobacco mature pollen and pollen activated in vitro.
Mol Cel Proteomics 15: 1338-1350. doi: 10.1074/mcp.M115.051672. PDF

Dupláková N, Dobrev PI, Reňák D, Honys D (2016) Rapid separation of Arabidopsis male gametophyte developmental stages using a Percoll gradient.
Nature Protocols 11(10): 1817-1832. PDF

Hafidh D, Fíla J, Honys D (2016) Male gametophyte development and function in angiosperms: a general concept.
Plant Reproduction 29: 31-51, doi: 10.1007/s00497-015-0272-4. PDF

Jurečková JF, Sýkorová E, Hafidh S, Honys D, Fajkus J, Fojtová M (2016) Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum.
Planta. Online first. DOI 10.1007/s00425-016-2624-1. PDF

Majerská J, Schrumpfová PP, Dokládal L, Schořová Š, Stejskal K, Obořil M, Honys D, Kozáková L, Polanská PS, Sýkorová E (2016) Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo.
Protoplasma. Online first. DOI 10.1007/s00709-016-1042-3. PDF

Hofer M, Falk M, Komůrková D, Falkova I, Bačíková A, Klejdus B, Pagáčová E, Štefančíková L, Weiterova L, Angelis KJ, Kozubek S, Dušek L, Galbavý Š (2016) Two New Faces of Amifostine: Protector from DNA Damage in Normal Cells and Inhibitor of DNA Repair in Cancer Cells.
Journal of Medicinal Chemistry 59: 3003-3017. DOI: 10.1021/acs.jmedchem.5b01628. PDF

Rollo A, Lojka B, Honys D, Mandák B, Chia Wong JA, Santos C, Costa R, Quintela-Sabarís C, Ribeiro MM (2016) Genetic diversity and hybridization in the two species Inga ingoides and Inga edulis: potential applications for agroforestry in the Peruvian Amazon. Annals of Forest Science 73: 425-435. doi: 10.1007/s13595-015-0535-0. PDF

Balážová A, Kolář F, Fíla J, Mikát M, Baláž V (2016)
Rozmnožování z pohledu evoluce. Námluvy, sňatky a podvody v říši živočichů a rostlin. Academia. Praha. ISBN 978-80-200-2590-6.




2015


Bokvaj P, Hafidh S, Honys D (2015) Transcriptome profiling of male gametophyte development in Nicotiana tabacum.
Genomics Data 3: 106-111. PDF

Dokládal L, Honys D, Rana R, Lee L-Y, Gelvin S, Sýkorová E (2015) cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-telomeric Roles of Arabidopsis Telomerase.
Front Plant Sci 6:985. PDF

Fojtová M, Sýkorová E, Najdekrová L, Polanská P, Zachová D, Vágnerová R, Angelis KJ, Fajkus J (2015) Telomere dynamics in the lower plant Physcomitrella patens.
Plant Mol Biol 87: 591-601. PDF

Furukawa T, Angelis KJ, Britt AB (2015) Arabidopsis DNA polymerase lambda mutant is mildly sensitive to DNA double strand breaks but defective in integration of a transgene.
Front Plant Sci 6: 357. PDF

Holá M, Vágnerová R, Angelis KJ (2015) Mutagenesis during plant responses to UVB radiation.
Plant Physiol Biochem 93: 29-33. PDF




2014


Reňák D, Gibalová A, Šolcová K, Honys D (2014) A new link between stress response and nucleolar function during pollen development in Arabidopsis mediated by AtREN1 protein.
Plant Cell & Environ 37: 670-683. PDF

Fíla J, Čapková V, Honys D (2014) Phosphoproteomic studies in Arabidopsis and tobacco male gametophytes.
Biochem Soc Trans 42: 383-387. PDF

Hafidh S, Potěšil D, Fíla J, Feciková J, Čapková V, Zdráhal Z, Honys D (2014) In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception.
Biochem Soc Trans 42: 388-394. PDF

da Costa-Nunes JA, Capitão C, Kozak J, Costa-Nunes P, Ducasa GM, Pontes O, Angelis KJ (2014) The AtRAD21.1 and AtRAD21.3 Arabidopsis cohesins play a synergistic role in somatic DNA double strand break damage repair.
BMC Plant Biol 14: 353. PDF




2013


Ding Z, Wang B, Moreno I, Dupľáková N, Simon S, Carraro N, Reemmer J, Pěnčík A, Chen X, Tejos R, Skůpa P, Pollmann S, Mravec J, Petrášek J, Zažímalová E, Honys D, Rolčík J, Murphy A, Orellana A, Geisler M, Friml J (2013) Corrigendum: ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis.
Nature Communications 4:1421. DOI:10.1038/ncomms2452.

Pičmanová M, Reňák D, Feciková J, Růžička P, Mikšátková P, Lapčík O, Honys D (2013) Functional expression and subcellular localization of pea polymorphic isoflavone synthase CYP93C18.
Biol Plant 57(4): 635-645.

Hafidh S, Potěšil D, Zdráhal Z, Honys D (2013) DEAD-box helicases are among the constituents of the tobacco pollen mRNA storing bodies.
J Plant Biochem Physiol 1:114. doi: 10.4172/2329-9029.1000114.

Holá M, Kozák J, Vágnerová R, Angelis KJ (2013) Genotoxin Induced Mutagenesis in the Model PlantPhyscomitrella patens.
BioMed Res Int 2013:535049.

Fíla J, Kodejš K, Mikát M, Nunvář J, Smyčka J, Synek P, Zouhar (2013)
Komunikace. Učebnice biologie. Ed. Jan Fíla. Česká zemědělská univerzita v Praze. ISBN 978-80-213-2386-5.




2012


Fíla J, Matros A, Radau S, Peiman Zahedi R, Čapková V, Mock H-P, Honys D (2012) Revealing phosphoproteins playing role in tobacco pollen activated in vitro.
Proteomics 12(21): 3229-3250.

Ding Z, Wang B, Moreno I, Dupľáková N, Simon S, Carraro N, Reemmer J, Pěnčík A, Chen X, Tejos R, Skůpa P, Pollmann S, Mravec J, Petrášek J, Zažímalová E, Honys D, Rolčík J, Murphy A, Orellana A, Geisler M, Friml J (2012) ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis.
Nature Communications 3:941, doi: 10.1038/ncomms1941.

Hafidh S, Breznenová K, Růžička P, Feciková J, Čapková V, Honys D (2012) Comprehensive analysis of tobacco pollen transcriptome unveils common pathways in polar-cell expansion and underlying heterochronic shift during spermatogenesis.
BMC Plant Biology 12:24.

Hafidh S, Breznenová K, Honys D (2012) De novo post-pollen mitosis II tobacco pollen tube transcriptome.
Plant Signaling Behavior 7(8): 918-921.

Reňák D, Dupľáková N, Honys D (2012) Wide-scale screening of T-DNA lines for transcription factor genes affecting male gametophyte development in Arabidopsis.
Sex Plant Reprod 25(1): 39-60.

Fíla J, Honys D (2012) Enrichment techniques employed in phosphoproteomics.
Amino Acids 43: 1025-1047.

Kamisugi Y, Schaefer DG, Kozák J, Charlot F, Vrielynck N, Holá M, Angelis KA, Cuming AC, Nogué F (2012) MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens.
Nucleic Acids Res 40(8): 3496-3510.

Pičmanová M, Koblovská R, Lapčík O, Honys D (2012) Isoflavone synthase genes in legumes and non-leguminous plants. 2012
ICBEB: 344-347.

Fíla J, Matros A, Čapková V, Mock H-P, Honys D (2012) Specificity of MOAC enrichment applied for mature pollen phosphoproteomic studies. 2012
ICBEB: 523-526.

Baláž V, Balážová A, Fíla J, Kolář F, Mikát M (2012)
Láska, sex a něžnosti v říši živočichů a rostlin. Učebnice biologie. Ed. Alena Balážová. Česká zemědělská univerzita v Praze. ISBN 978-80-213-2288-2.




2011


Böhmdorfer G, Schleiffer A, Brunmeir R, Ferscha S, Nizhynska V, Kozák J, Angelis KJ, Kreil DP, Schweizer D (2011) GMI1, a structural-maintenance-of-chromosomes-hinge domain-containing protein, is involved in somatic homologous recombination in Arabidopsis.
Plant J 67: 420-433.

Hafidh S, Čapková V, Honys D (2011) Safe keeping the message: mRNP complexes tweaking after transcription.
Adv Exp Med Biol 722: 118-136.

Fíla J, Čapková V, Feciková J, Honys D (2011) Impact of homogenization and protein extraction conditions on the obtained tobacco proteomic pattern.
Biol Plant 55(3): 499-506.

Szabová J, Růžička P, Verner Z, Hampl V, Lukeš J (2011) Experimental Examination of EFL and MATX Eukaryotic Horizontal Gene Transfers: Coexistence of Mutually Exclusive Transcripts Predates Functional Rescue.
Mol Biol Evol 28(8): 2371–2378.

Fidlerová AA, Honys D (2011) Jan Evangelista Purkyně (nejen) slezským rostlinozpytcem.
Živa 59(5): 214-217.

Fíla J, Pánek T, Sekereš J (2011)
Tvary v živé přírodě. Učebnice biologie. Ed. Jan Fíla. Česká zemědělská univerzita v Praze. ISBN 978-80-213-2191-5.




2010


Šmídková M, Müller M, Thönes N, Piuko K, Angelisová P, Velemínský J, Angelis KJ (2010) Transient expression of human papillomavirus type 16 virus-like particles in tobacco and tomato using a tobacco rattle virus expression vector.
Biol Plant 54(3) 451-460.

Šmídková M, Holá M, Angelis KJ (2010) Efficient biolistic transformation of the moss Physcomitrella patens.
Biol Plant 54(4): 777-780.




2009


Gibalová A, Reňák D, Matczuk K, Dupľáková N, Cháb D, Twell D, Honys D (2009) AtbZIP34 is required for Arabidopsis pollen wall patterning and the control of several metabolic pathways in developing pollen.
Plant Molecular Biology 70(5): 581-601.

Honys D, Reňák D, Feciková J, Jedelský PL, Nebesářová J, Dobrev P, Čapková V (2009) Cytoskeleton-associated large RNP complexes in tobacco male gametopyte (EPPs) are associated with ribosomes and are involved in protein synthesis, processing and localisation.
Journal of Proteome Research 8(4): 2015-2031.

Kozák J, West CE, White C, da Costa-Nunes JA, Angelis KJ (2009) Rapid repair of DNA double strand breaks in Arabidopsis thaliana is dependent on proteins involved in chromosome structure maintenance.
DNA Repair 8: 413-419.

Waterworth W, Kozák J, Provost CM, Bray CM, Angelis KJ, West CE (2009) DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks.
BMC Plant Biol 9: 79.




2008


Hoelzl C, Glatt H, Meinl W, Sontag G, Haidinger G, Kundi M, Simic T, Chakraborty A, Bichler J, Ferk F, Angelis KJ, Nersesyan A, Knasmüller S (2008) Consumption of Brussels sprouts protects peripheral human lymphocytes against 2-amino-1- methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and oxidative DNA-damage: results of a controlled human intervention trial.
Mol Nutr Food Res 52: 330-341.

Honys D, Dupľáková N, Reňák D (2008) On Line Tools for Presentation and Analysis of Plant Microarray Data. In Martina K. Moretti and Lorenzo J. Rizzo (eds.)
Oligonucleotide Array Sequence Analysis, Nova Publishers, ISBN 978-1-60456-542-3, pp 265-295.

Kosová K, Holková L, Prášil IT, Prášilová P, Bradáčova M, Vitámvás P, Čapková V (2008) Expression of dehydrin 5 during the development of frost tolerance in barley (Hordeum vulgare)
J Plant Physiol 165 (11): 1142-1151.




2007


Bichler J, Cavin C, Simic T, Chakraborty A, Ferk F, Hoelzl C, Schulte-Hermann R, Kundi M, Haidinger G, Angelis K, Knasmüller S (2007) Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers.
Food Chem Toxicol 45: 1428-1436.

Dupľáková N, Reňák D, Hovanec P, Honysová B, Twell D, Honys D (2007) Arabidopsis Gene Family Profiler (aGFP) - user-oriented transcriptomic database with easy-to-use graphic interface
BMC Plant Biology 7:39.

Vitámvás P, Saalbach G, Prášil IT, Čapková V, Opatrná J, Ahmed J (2007) WCS120 protein family and proteins soluble upon boiling in cold-acclimated winter wheat.
J Plant Physiol 164(9): 1197-1207.




2006


Honys D, Oh SA, Reňák D, Johnson A, Šolcová B, Boudová R, Twell D (2006) Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis.
BMC Plant Biology 6(1):31.

Bock KW, Honys D, Ward JM, Padmanaban S, Nawrocki EP, Hirschi KD, Twell D, Sze H (2006) Integrating membrane transport with male gametophyte development and function through transcriptomics.
Plant Physiol 140: 1151-1168.

Novak, JP, Kim SY, Xu J, Modlich O, Volsky DJ, Honys D, Slonczewski JL, Bell DA, Blattner FR, Blumwald E, Boerma M, Cosio M, Gatalica Z, Hajduch M, Hidalgo J, McInnes RR, Miller MC, Penkowa M, Rolph M, Sottosanto J, St. Arnaud R, Szego MJ, Twell D, Wang C (2006) Generalization of DNA microarray dispersion properties: microarray equivalent of t-distribution.
Biology Direct 1:27.

Lapčík O, Honys D, Koblovská R, Macková Z, Vítková M, Klejdus B (2006) Isoflavonoids are present in Arabidopsis thaliana despite the absence of any homologue to known isoflavonoid synthases.
Plant Physiol Biochem 44: 106-114.

Honys D, Reňák D, Twell D (2006) Male gametophyte development and function. In: Jaime Teixeira da Silva (ed.)
Floriculture, Ornamental and Plant Biotechnology: advances and topical issues (1st Edition), Global Science Books, London, 76-87.

Twell D, Oh SA, Honys D (2006) Pollen development, a genetic and transcriptomic view. In: Rui Malhó (ed.)
Plant Cell Monographs vol. 3, The Pollen Tube. Springer-Verlag Berlin, Heidelberg, ISBN 3540311211, pp 15-45.




2005


Hrubá P, Honys D, Twell D, Čapková V, Tupý J (2005) Expression of genes for beta-galactosidases and beta-xylosidases during microspore and pollen development.
Planta 220 (6): 931-940.

Hájek T, Honys D, Witters E, Čapková V (2005) Identification of stress-induced mitochondrial proteins in cultured tobacco cells.
Physiol Plant 124: 12-24.

Hrubá P, Honys D, Tupý J (2005) Expression and thermotolerance of calreticulin during pollen development in tobacco.
Sex Plant Reprod 18: 143-148.




2004


Hájek T, Honys D, Čapková V (2004) New method of plant mitochondria isolation and sub-fractionation for proteomic analyses.
Plant Sci 167: 389-395.

Heitzeberg F, Chen I-P, Hartung F, Orel N, Angelis KJ, Puchta H (2004) The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination.
Plant J 38: 954-968.

Honys D, Twell D (2004) Transcriptome analysis of developing haploid male gametophytes in Arabidopsis thaliana.
Genome Biology 5: R85.

Honys D, Twell D (2004) Male gametogenesis In: R.M. Goodman (ed.)
Encyclopedia of Plant & Crop Science, Marcel Dekker Inc., New York, ISBN 0824709446.

Lalanne E, Honys D, Johnson A, Borner G, Dupree P, Grossniklaus U, Twell D (2004) AtPIGC and AtPIGA, two components of the glycosylphosphatidylinositol anchor biosynthetic pathway, are required for pollen germination and tube growth in Arabidopsis.
Plant Cell 16: 229-240.

Pokorná J, Schwarzerová K, Zelenková S, Petrášek J, Janotová I, Čapková V, Opatrný Z (2004) Sites of actin filament initiation and reorganization in cold-treated tobacco cells.
Plant Cell & Environ 27: 641-653.

Sze H, Padmanaban S, Cellier F, Honys D, Cheng NH, Bock KW, Conéjero G, Li X, Twell D, Ward JM, Hirschi KD (2004) Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development.
Plant Physiol 136: 2532-2547.

Takeda S, Tadele Z, Hofmann I, Probst AV, Angelis KJ, Kaya H, Araki T, Mengiste T, Mittelsten Scheid O, Shibahara K, Scheel D, Paszkowski J (2004) BRU1, a novel link between responses to DNA damage and epigenetic gene silencing in Arabidopsis.
Genes Dev 18: 782-793.




2003


Honys D, Twell D (2003) Comparative analysis of Arabidopsis pollen transcriptome.
Plant Physiol 132: 640-652.

Motyka V, Vaňková R, Čapková V, Petrášek J, Kamínek M, Schmülling T (2003) Citokinin-induced upregulation of cytokinin oxidase activity in tobacco includes changes in enzyme glycosylation and secretion.
Physiol Plant 1117: 11-21.

Schwarzerová K, Pokorná J, Petrášek J, Zelenková S, Čapková V, Janotová I, Opatrný Z (2003) The structure of cortical cytoplasm in cold-treated tobacco cells. The role of the cytoskeleton and the endomembrane system.
Cell Biol Int 27:263-265.




2002


Hartung F, Angelis KJ, Meister A, Schubert I, Melzer M, Puchta H (2002) An archaebacterial topoisomerase homolog not present in other eukaryotes is indispensable for cell proliferation of plants.
Curr Biol 12: 1787-1791.




2001


Fidlerová A, Smýkal P, Tupý J, Čapková V (2001) Glycoproteins 66 and 69 kDa of pollen tube wall: properties and distribution in Angiosperms.
J Plant Physiol 158: 1367-1374.

Haisel D, Hofman P, Vágner M, Lipavská H, Tichá I, Schäfer C, Čapková V (2001) Ex vitro phenotype stability is affected by in vitro cultivation.
Biol Plant 44: 321-324.

Honys D (2001) Isolation of proteins comprising native gene-specific messenger ribonucleoprotein particles using paramagnetic beads.
Plant Sci 161: 605-611.

Kadleček P, Tichá I, Haisel D, Čapková V, Schäfer Ch (2001) Importance of in vitro pretreatment for ex vitro acclimation and growth.
Plant Sci 161: 695-701.

Menke M, Chen I-P, Angelis KJ, Schubert I (2001) DNA damage and repair in Arabidopsis thaliana as measured by the comet assay after treatment with different classes of genotoxins.
Mutation Res 493: 87-93.




2000


Angelis KJ, McGuffie M, Menke M, Schubert I (2000) Adaptation to alkylation damage in DNA measured by the comet assay.
Environ Mol Mutagenesis 36: 146-150.

Honys D, Combe JP, Twell D, Čapková V (2000) The translationally repressed pollen-specific ntp303 mRNA is stored in non-polysomal mRNPs during pollen maturation.
Sex Plant Reprod 13: 135-144.

Honys D, Čapková V (2000) Temporal changes in the RNA distribution between polysomes and postpolysomal ribonucleoprotein particles in tobacco male gametophyte.
Biol Plant 43(4): 517-522.

Mašek T, Smýkal P, Janotová I, Honys D, Čapková V, Pechan PM (2000) Isolation of a Brassica napus L. cDNA encoding a putative high-mobility-group HMG I/Y protein.
Plant Sci 159: 197-204.

Menke M, Angelis KJ, Schubert I (2000) Detection of specific DNA lesions by a combination of comet assay and FISH in plants.
Environ Mol Mutagenesis 35: 132-138.

Wittink FRA, Knuiman B, Derksen J, Čapková V, Twell D, Schrauwen JAM, Wullems GJ (2000) The pollen-specific gene ntp303 encodes a 69-kDa glycoprotein associated with the vegetative membrane and the cell wall.
Sex Plant Reprod 12: 276-284.